In the Crooks Lab we are interested in how human hematopoietic stem cells generate the immune system during development and after transplantation. Our work has evolved into three complementary areas of study:

 

1. Lineage commitment in human stem and lymphoid progenitor populations in bone marrow, cord blood and thymus

2. Regulation of hematopoiesis from human pluripotent stem cells (hPSC)

3. Development of the thymic microenvironment 

 

To study these areas we use a range of methods including multi-parameter flow cytometry, a variety of in vitro differentiation systems and transplantation models, high throughput sequencing of RNA and DNA, lentiviral vector-mediated gene expression and clonal tracking, in vivo imaging and bioengineering.

 

 

The goal of our work is to develop new ways to generate and regulate 

a healthy immune system from hematopoietic stem cells.