Selected publications

Gensheimer J, LaGosh J, Moulton ER, Sun V, de Barros SC, Montecino-Rodriguez E, Yiu G, Yuan X, Dorshkind K, Crooks GM. Clonal Analyses Reveal the Impact of Hematopoietic Stem and Progenitor Cell Aging on T Cell Development. Aging Cell. 2026 Jul;25(7):e70615. doi: 10.1111/acel.70615. PMID: 42374656; PMCID: PMC13314711.

Sang Pil Yoo, Xuegang Yuan, Claire Engstrom, Patrick Chang, Suwen Li, Lindsay Lathrop, Jessica Lagosh, Satvik Saripalli, Anthony Azzun, Vincenzo Calvanese, Hanna Mikkola, Christopher Seet, Donald B. Kohn, Gay M. Crooks. Stage-specific CAR-mediated signaling generates naïve-like, TCR-null CAR T cells from induced pluripotent stem cells. 2024.11.25.624041; doi: https://doi.org/10.1101/2024.11.25.624041.

Chang, P. C., Yuan, X., Zampieri, A., Towns, C., Yoo, S. P., Engstrom, C., Tsai, S., Robles, C. R., Zhu, Y., Lopez, S., Montel-Hagen, A., Seet, C. S., & Crooks, G. M. (2024). Generation of antigen-specific mature T cells from RAG1-/-RAG2-/-B2M-/- stem cells by engineering their microenvironment. Nature biomedical engineering, 8(4), 461–478. https://doi.org/10.1038/s41551-023-01146-7.

McAuley G, Yiu G, Chang PC, Newby GA, Campo-Fernandez B, Fitz-Gibbon ST, Wu X, Kang SL, Garibay A, Butler J, Christian V, Wong RL, Everette KA, Azzun A, Gelfer H, Seet CS, Narendran A, Murguia-Favela L, Romero Z, Wright N, Liu DR, Crooks GM, Kohn DB. Human T cell generation is restored in CD3δ severe combined immunodeficiency through adenine base editing. Cell. 2023 Mar 30. doi:10.1016/j.cell.2023.02.027.

Li S, Wang CS, Montel-Hagen A, Chen HC, Lopez S, Zhou O, Dai K, Tsai S, Satyadi W, Botero C, Wong C, Casero D, Crooks GM, Seet CS. Strength of CAR signaling determines T cell versus ILC differentiation from pluripotent stem cells. Cell Rep. 2023 Mar 28. doi:10.1016/j.celrep.2023.112241.

Montel-Hagen, A., Tsai, S., Seet, C. S., & Crooks, G. M. (2022). Generation of Artificial Thymic Organoids from Human and Murine Hematopoietic Stem and Progenitor Cells. Current protocols, 2(4), e403. https://doi.org/10.1002/cpz1.403.

Sun, V., Sharpley, M., Kaczor-Urbanowicz, K. E., Chang, P., Montel-Hagen, A., Lopez, S., Zampieri, A., Zhu, Y., de Barros, S. C., Parekh, C., Casero, D., Banerjee, U., & Crooks, G. M. (2021). The Metabolic Landscape of Thymic T Cell Development In Vivo and In Vitro. Frontiers in immunology, 12, 716661. https://doi.org/10.3389/fimmu.2021.716661.

Montel-Hagen A, Sun V, Casero D, Tsai S, Zampieri A, Jackson N, Li S, Lopez S, Zhu Y, Chick B, He C, de Barros SC, Seet CS, Crooks GM. In Vitro Recapitulation of Murine Thymopoiesis from Single Hematopoietic Stem Cells. Cell Rep. 2020 Oct 27;33(4):108320. doi: 10.1016/j.celrep.2020.108320.

de Barros, S. C., Suterwala, B. T., He, C., Ge, S., Chick, B., Blumberg, G. K., Kim, K., Klein, S., Zhu, Y., Wang, X., Casero, D., & Crooks, G. M. (2020). Pleiotropic Roles of VEGF in the Microenvironment of the Developing Thymus. Journal of immunology (Baltimore, Md. : 1950), 205(9), 2423–2436. https://doi.org/10.4049/jimmunol.1901519.

Montel-Hagen, A., Seet, C. S., Li, S., Chick, B., Zhu, Y., Chang, P., Tsai, S., Sun, V., Lopez, S., Chen, H. C., He, C., Chin, C. J., Casero, D., & Crooks, G. M. (2019). Organoid-Induced Differentiation of Conventional T Cells from Human Pluripotent Stem Cells. Cell stem cell, 24(3), 376–389.e8. https://doi.org/10.1016/j.stem.2018.12.011.

Montel-Hagen, A., & Crooks, G. M. (2019). From pluripotent stem cells to T cells. Experimental hematology, 71, 24–31. https://doi.org/10.1016/j.exphem.2018.12.001.

Calvanese, V., Nguyen, A. T., Bolan, T. J., Vavilina, A., Su, T., Lee, L. K., Wang, Y., Lay, F. D., Magnusson, M., Crooks, G. M., Kurdistani, S. K., & Mikkola, H. K. A. (2019). MLLT3 governs human haematopoietic stem-cell self-renewal and engraftment. Nature, 576(7786), 281–286. https://doi.org/10.1038/s41586-019-1790-2.

Chin, C. J., Li, S., Corselli, M., Casero, D., Zhu, Y., He, C. B., Hardy, R., Péault, B., & Crooks, G. M. (2018). Transcriptionally and Functionally Distinct Mesenchymal Subpopulations Are Generated from Human Pluripotent Stem Cells. Stem cell reports, 10(2), 436–446. https://doi.org/10.1016/j.stemcr.2017.12.005.

Seet, C. S., He, C., Bethune, M. T., Li, S., Chick, B., Gschweng, E. H., Zhu, Y., Kim, K., Kohn, D. B., Baltimore, D., Crooks, G. M., & Montel-Hagen, A. (2017). Generation of mature T cells from human hematopoietic stem and progenitor cells in artificial thymic organoids. Nature methods, 14(5), 521–530. https://doi.org/10.1038/nmeth.4237.

Chin, C. J., Cooper, A. R., Lill, G. R., Evseenko, D., Zhu, Y., He, C. B., Casero, D., Pellegrini, M., Kohn, D. B., & Crooks, G. M. (2016). Genetic Tagging During Human Mesoderm Differentiation Reveals Tripotent Lateral Plate Mesodermal Progenitors. Stem cells (Dayton, Ohio), 34(5), 1239–1250. https://doi.org/10.1002/stem.2351.

Casero, D., Sandoval, S., Seet, C. S., Scholes, J., Zhu, Y., Ha, V. L., Luong, A., Parekh, C., & Crooks, G. M. (2015). Long non-coding RNA profiling of human lymphoid progenitor cells reveals transcriptional divergence of B cell and T cell lineages. Nature immunology, 16(12), 1282–1291. https://doi.org/10.1038/ni.3299.

Kim, W. S., Zhu, Y., Deng, Q., Chin, C. J., He, C. B., Grieco, A. J., Dravid, G. G., Parekh, C., Hollis, R. P., Lane, T. F., Bouhassira, E. E., Kohn, D. B., & Crooks, G. M. (2014). Erythropoiesis from human embryonic stem cells through erythropoietin-independent AKT signaling. Stem cells (Dayton, Ohio), 32(6), 1503–1514. https://doi.org/10.1002/stem.1677.

Chung, B., Montel-Hagen, A., Ge, S., Blumberg, G., Kim, K., Klein, S., Zhu, Y., Parekh, C., Balamurugan, A., Yang, O. O., & Crooks, G. M. (2014). Engineering the human thymic microenvironment to support thymopoiesis in vivo. Stem cells (Dayton, Ohio), 32(9), 2386–2396. https://doi.org/10.1002/stem.1731.

Kohn, L. A., Seet, C. S., Scholes, J., Codrea, F., Chan, R., Zaidi-Merchant, S., Zhu, Y., De Oliveira, S., Kapoor, N., Shah, A., Abdel-Azim, H., Kohn, D. B., & Crooks, G. M. (2014). Human lymphoid development in the absence of common γ-chain receptor signaling. Journal of immunology (Baltimore, Md. : 1950), 192(11), 5050–5058. https://doi.org/10.4049/jimmunol.1303496

Prashad, S. L., Calvanese, V., Yao, C. Y., Kaiser, J., Wang, Y., Sasidharan, R., Crooks, G., Magnusson, M., & Mikkola, H. K. (2015). GPI-80 defines self-renewal ability in hematopoietic stem cells during human development. Cell stem cell, 16(1), 80–87. https://doi.org/10.1016/j.stem.2014.10.020.

Corselli, M., Chin, C. J., Parekh, C., Sahaghian, A., Wang, W., Ge, S., Evseenko, D., Wang, X., Montelatici, E., Lazzari, L., Crooks, G. M., & Péault, B. (2013). Perivascular support of human hematopoietic stem/progenitor cells. Blood, 121(15), 2891–2901. https://doi.org/10.1182/blood-2012-08-451864.

Evseenko, D., Latour, B., Richardson, W., Corselli, M., Sahaghian, A., Cardinal, S., Zhu, Y., Chan, R., Dunn, B., & Crooks, G. M. (2013). Lysophosphatidic acid mediates myeloid differentiation within the human bone marrow microenvironment. PloS one, 8(5), e63718. https://doi.org/10.1371/journal.pone.0063718.

Parekh, C., & Crooks, G. M. (2013). Critical differences in hematopoiesis and lymphoid development between humans and mice. Journal of clinical immunology, 33(4), 711–715. https://doi.org/10.1007/s10875-012-9844-3.

Kohn, L. A., Hao, Q. L., Sasidharan, R., Parekh, C., Ge, S., Zhu, Y., Mikkola, H. K., & Crooks, G. M. (2012). Lymphoid priming in human bone marrow begins before expression of CD10 with upregulation of L-selectin. Nature immunology, 13(10), 963–971. https://doi.org/10.1038/ni.2405.

Parekh, C., Sahaghian, A., Kim, W., Scholes, J., Ge, S., Zhu, Y., Asgharzadeh, S., Hollis, R., Kohn, D., Ji, L., Malvar, J., Wang, X., & Crooks, G. (2012). Novel pathways to erythropoiesis induced by dimerization of intracellular C-Mpl in human hematopoietic progenitors. Stem cells (Dayton, Ohio), 30(4), 697–708. https://doi.org/10.1002/stem.1046.

Cuddihy, A. R., Suterwala, B. T., Ge, S., Kohn, L. A., Jang, J., Andrade, J., Wang, X., & Crooks, G. M. (2012). Rapid thymic reconstitution following bone marrow transplantation in neonatal mice is VEGF-dependent. Biology of blood and marrow transplantation : journal of the American Society for Blood and Marrow Transplantation, 18(5), 683–689. https://doi.org/10.1016/j.bbmt.2012.01.006

Andrade, J., Ge, S., Symbatyan, G., Rosol, M. S., Olch, A. J., & Crooks, G. M. (2011). Effects of sublethal irradiation on patterns of engraftment after murine bone marrow transplantation. Biology of blood and marrow transplantation : journal of the American Society for Blood and Marrow Transplantation, 17(5), 608–619. https://doi.org/10.1016/j.bbmt.2010.12.697

Dravid, G., Zhu, Y., Scholes, J., Evseenko, D., & Crooks, G. M. (2011). Dysregulated gene expression during hematopoietic differentiation from human embryonic stem cells. Molecular therapy : the journal of the American Society of Gene Therapy, 19(4), 768–781. https://doi.org/10.1038/mt.2010.281

Evseenko, D., Zhu, Y., Schenke-Layland, K., Kuo, J., Latour, B., Ge, S., Scholes, J., Dravid, G., Li, X., MacLellan, W. R., & Crooks, G. M. (2010). Mapping the first stages of mesoderm commitment during differentiation of human embryonic stem cells. Proceedings of the National Academy of Sciences of the United States of America, 107(31), 13742–13747. https://doi.org/10.1073/pnas.1002077107

Cuddihy, A. R., Ge, S., Zhu, J., Jang, J., Chidgey, A., Thurston, G., Boyd, R., & Crooks, G. M. (2009). VEGF-mediated cross-talk within the neonatal murine thymus. Blood, 113(12), 2723–2731. https://doi.org/10.1182/blood-2008-06-162040

Evseenko, D., Schenke-Layland, K., Dravid, G., Zhu, Y., Hao, Q. L., Scholes, J., Wang, X. C., Maclellan, W. R., & Crooks, G. M. (2009). Identification of the critical extracellular matrix proteins that promote human embryonic stem cell assembly. Stem cells and development, 18(6), 919–928. https://doi.org/10.1089/scd.2008.0293

Abdel-Azim, H., Zhu, Y., Hollis, R., Wang, X., Ge, S., Hao, Q. L., Smbatyan, G., Kohn, D. B., Rosol, M., & Crooks, G. M. (2008). Expansion of multipotent and lymphoid-committed human progenitors through intracellular dimerization of Mpl. Blood, 111(8), 4064–4074. https://doi.org/10.1182/blood-2007-08-107466

Hao, Q. L., George, A. A., Zhu, J., Barsky, L., Zielinska, E., Wang, X., Price, M., Ge, S., & Crooks, G. M. (2008). Human intrathymic lineage commitment is marked by differential CD7 expression: identification of CD7- lympho-myeloid thymic progenitors. Blood, 111(3), 1318–1326. https://doi.org/10.1182/blood-2007-08-106294